FastQCFastQC Report
Wed 20 Jul 2016
Undetermined_reverse_paired.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameUndetermined_reverse_paired.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences483674
Sequences flagged as poor quality0
Sequence length50-241
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC12590.26029929249866646No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTGGAC1100.001738389137.434153
TCAGGAC1400.00566647629.4125463
GTATAGA2605.3621734E-627.7156721
CTCTAGT1907.66977E-427.0905021
GTACTGG1550.00931276726.5661741
GGACCAT1958.9262164E-426.3958766
AGGACCA2000.001034758825.7359815
TTACACT2809.535574E-625.7359774
ATCCTAG2100.001375226324.5104561
GTGTATG2551.495281E-424.2220971
CTATAAG2200.001802993523.3963453
ACCGATG2200.001802993523.3963458
TATAGCG2200.001802993523.3963455
TAGTCCA2300.002334670622.3791124
GTCTTAA2350.002645247421.902961
CAGGACA2853.1756313E-421.6724034
TAGGAGG2853.1756313E-421.6724036
TAGAACC2550.00424720920.1850814
GCTATAC2550.00424720920.1850813
ACAGCTC2600.004752299319.7969078