FastQCFastQC Report
Wed 20 Jul 2016
Undetermined_forward_paired.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameUndetermined_forward_paired.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences483674
Sequences flagged as poor quality0
Sequence length50-241
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTAGAC1007.8224204E-444.0336843
TAAGGGT1705.9365684E-638.8532524
CTCTAAG1702.7001408E-432.377711
AAGGGTG1400.004081992431.452635
CCTTATA2851.8626815E-730.9008271
CTACACT1905.1817385E-428.9695284
GTGCTAG1550.006713940328.4088271
TCTACAC3154.4742046E-727.9578913
ACTATCC2805.66418E-627.5210518
TATGACA3756.942537E-826.420214
TATACTG3802.2960558E-623.1756235
GTATAAC3452.8557688E-522.3359261
CTTACAC2600.003222741421.1700383
TATTGTG3704.8984228E-520.8267425
TAAGACT2650.003598969720.7706034
ATACAGA5951.2838427E-820.3517026
CTAAACT3856.651093E-520.015314
TTCTAGG3356.0113746E-419.7165742
GTATCGC2800.004949994419.6578941
CCTATAT2800.004949994419.6578941