FastQCFastQC Report
Wed 20 Jul 2016
PSAE004-NC4_reverse_paired.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenamePSAE004-NC4_reverse_paired.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1524500
Sequences flagged as poor quality0
Sequence length50-241
%GC33

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTGCG1551.9518858E-434.223369
CCGTGCT2357.004086E-527.0874239
ACCGTGC4558.894858E-1025.6487128
GACCGTG6409.094947E-1121.550027
TAGGCCT2600.00399576720.4023864
TATACTG23050.019.7916415
GGAGAGC2700.00497107919.646746
TAGACTC6703.7143764E-919.0016255
CTAGCCT2800.006133729618.9450724
TCTAGCC4101.4337522E-418.1133383
TACACCG10050.017.9459785
ACAGTCC4151.5731758E-417.8951058
TAGGCAG3600.001251436117.6820685
CCTCTAC4953.1082192E-517.1462481
GTATATA34250.016.417221
GTCTAGA7151.6108243E-716.3219091
AGACCGT7251.8865467E-716.0967796
ATAAGGG10902.7284841E-1115.5731973
AAGACCG6851.8456303E-615.4879435
ACAGAGC6251.3304274E-515.2773068