FastQCFastQC Report
Wed 20 Jul 2016
PSAE004-NC4_forward_paired.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenamePSAE004-NC4_forward_paired.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1524500
Sequences flagged as poor quality0
Sequence length50-241
%GC33

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGAGCG600.00559949155.4524429
GAGGCCA1450.004676784430.594459
ACTGTGC8450.020.9997438
TAGGGTG4807.0207716E-720.7946665
AGACCCT4407.689905E-620.1645246
TACTCCG2850.005257986519.4569975
GTCCTAG4651.2392207E-519.0804121
TCCTACC4151.1198723E-418.7068482
CCCTATG3150.00936689717.603959
GCTAGAC5102.7441889E-517.3968453
TATACAG23050.017.3213715
TATACCG11800.016.9176945
AACCGAG4000.001882687916.6357337
GGACTGA4000.001882687916.6357336
ACTGGGC4050.00204568916.4303538
TAGACAG7451.5521255E-716.3752175
TACCGTG7652.0979314E-715.9471087
CTTATAC17650.015.7089073
ATAAGGG11302.3646862E-1115.7033463
GTGACCT5004.6462644E-415.5266854