FastQCFastQC Report
Wed 20 Jul 2016
MG319544_reverse_paired.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameMG319544_reverse_paired.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences682350
Sequences flagged as poor quality0
Sequence length50-241
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCCCCG500.002837361365.832918
CACCGGG1400.0041496431.3490077
TAGACTG3302.0745574E-829.924055
GTATATA7450.027.9826681
GTATAGG4854.1836756E-1127.1475931
TATACCG5251.1641532E-1025.0792035
AGACTGT6351.8189894E-1224.190576
ACTGTGC2952.6067984E-422.316248
GTATTAG12950.022.029031
TAGACCG3002.9206395E-421.9443025
GTATAAG7801.8189894E-1221.1002921
ACCGTCC2600.00328636721.100298
ACCGTAC2650.003669974220.7021718
CGTACAC2750.004547868419.9493643
CCTATAC4459.289677E-619.7252163
ACAGGTC4009.157774E-519.2012658
GTCTTAC4601.236692E-519.0820031
TAGGGGA2900.006182107618.9175034
CTATACC4651.3575149E-518.8768184
ACTGAGC3508.2579063E-418.80948