FastQCFastQC Report
Wed 20 Jul 2016
MG319544_forward_paired.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameMG319544_forward_paired.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences682350
Sequences flagged as poor quality0
Sequence length50-241
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGACCGG1152.4246629E-548.746266
GTGTGCA3052.8777868E-729.4075819
ATAGGAC3705.1111783E-827.2715573
GTATATA7100.026.8447741
GCTTAGG2957.376757E-626.6038911
TATAGGA5709.094947E-1225.5704062
TATACAG9300.025.3166075
TAGACAG5101.7571438E-924.1819675
GTATAGG4852.7319402E-823.1167831
GTCTAAG3402.2165876E-523.0827871
GTGCTAA6803.6379788E-1223.0827871
GTCTCAC2600.002899528221.5608461
GTATGTA4204.6736695E-621.3555051
CCTTAGA3704.257754E-521.211212
CAGGGCA3203.9038973E-421.0218269
CTATATA4856.9753696E-720.8051052
GTCTAGG2700.0036088220.7622972
AGGGCTG2700.0036088220.7622975
GTATACA5951.042099E-820.72744
TTATACT9350.020.38484