FastQCFastQC Report
Wed 20 Jul 2016
MG319031_reverse_paired.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameMG319031_reverse_paired.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences767208
Sequences flagged as poor quality0
Sequence length50-241
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTAGAC2654.0803734E-628.7098273
ATAGGGG2601.1809952E-425.0816633
CTAGGAC3201.763872E-523.7753263
AAGGGTC2350.00193063523.1249395
AGAGGGC1950.007409064622.2948118
CTTAGAG2952.7797202E-422.1058733
ACAGTGT5455.4224074E-921.9368678
GTATAGG5556.688424E-921.5416091
GTTACAC3553.9328974E-521.431283
TATAGGA6609.822543E-1121.4080872
TACAGGG3654.871829E-520.8441222
CTAGACT4307.49862E-620.2208774
GTATACT6001.6409103E-819.9259874
GTCTTAG6001.6409103E-819.9259871
TAGACGG4409.146577E-619.761315
CTATAGG3356.5559987E-419.4663661
CAGTCCG3356.5559987E-419.4663669
CAGTGCG2800.00533213319.408439
ACCGTGC2850.005906336519.0679328
GGTATAG5302.1994638E-618.4563181