FastQCFastQC Report
Wed 20 Jul 2016
MG319031_forward_paired.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameMG319031_forward_paired.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences767208
Sequences flagged as poor quality0
Sequence length50-241
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTAGG3207.2759576E-1238.6517261
TCTAGGG2702.3064786E-937.4804572
ACCGGGC1300.002560178734.5973478
ACAGGGC1652.002061E-434.0731438
GTGTGCA2507.180779E-526.9859319
CTAGGCT3008.220102E-626.2363224
GTCCTGG2159.434265E-426.1491581
TATACTG8250.025.895595
TACACCG8100.024.9869735
TGTAGGG3201.3560179E-524.5965542
GTGTAGG5151.902663E-924.0166031
TAGGCTG4953.2907337E-822.7154315
TAGACCG3002.4751868E-422.4882775
CGTATAC6505.0931703E-1122.4882763
TACACTG6308.3491614E-1021.4174045
ACTGTCC5306.781556E-821.2153558
CCTACAC5909.151336E-920.9636463
TTATACT9750.020.758414
GTATAAG6551.3569661E-920.5999471
GTACTAG5501.00282705E-720.4438861