FastQCFastQC Report
Wed 20 Jul 2016
MG318865_reverse_paired.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameMG318865_reverse_paired.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences865639
Sequences flagged as poor quality0
Sequence length50-241
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGGCC1150.001614938838.0031434
TAGGGCG1550.006968172728.1958795
AGGGCGC1650.009453231526.4870416
TAGACTG4258.8421075E-925.708015
GTATATA9850.025.5122641
CTAGTAC5801.6370905E-1124.4890943
GTCTTAG6850.023.9253391
ACAGTGC3802.454908E-623.0019048
CTAGACT4853.594687E-822.5276364
TATACTG10850.022.1539065
TGGACCG4954.461981E-822.0725355
GTGCATG4054.2674546E-621.5820331
CAGTGCC3053.361474E-421.4935849
AGCCCGT2550.003009820121.4233426
GTGGTCC2550.003009820121.4233428
GTAGCCC2600.003368557421.0113543
CTGTGCG2650.003761694320.6149149
GTGCTAG4808.096704E-720.486071
TACCCTA3255.160476E-420.1708985
TATATAC9250.020.080043