FastQCFastQC Report
Wed 20 Jul 2016
MG318865_forward_paired.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameMG318865_forward_paired.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences865639
Sequences flagged as poor quality0
Sequence length50-241
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCCTG2901.8281571E-730.9723055
CTGCGCG1500.00519727629.9398989
CCCTTAG3254.9571827E-727.6368261
CCTATAC5803.0195224E-1023.2292293
CACCGCA3902.4283763E-623.0306917
ACCGTCC3002.5005342E-422.4549228
ACCGGGA2550.002569913622.014638
CATAGGG2600.002876518821.591273
TATACCG5807.6470315E-921.293465
CCTTAGG2700.00358021920.7915941
AGTCCGC3254.294799E-420.727626
GTCCTAA6651.6698323E-920.2600781
CTGCGCA3355.268703E-420.1088859
CCCGCAC2800.004419523820.0490368
TAAGACC4559.2320715E-619.7405914
ACCACGG2850.00489606119.69735
CACACAG2850.00489606119.69735
CAGTCTA4007.67767E-519.6480569
CTAGACC5201.3437493E-619.4321444
GTAGCCT4109.281061E-519.1688354