FastQCFastQC Report
Wed 20 Jul 2016
MG317748_reverse_paired.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameMG317748_reverse_paired.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences470299
Sequences flagged as poor quality0
Sequence length50-241
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACCGTG1401.0676007E-437.9140857
CTAGGAC1250.002798995233.971023
ACCGTGC1652.8041808E-432.169538
TATACCG3308.039933E-1032.169535
CCTAGGA1550.008015322527.3959832
ACCGAGC1957.4581185E-427.220378
GTCCTAG2008.646797E-426.539861
TACCGAG4006.2827894E-926.539867
TGCACTG2909.860874E-625.6246915
GTATAGC4251.1979864E-824.9786911
ACTATAC3101.6534139E-523.9714853
TAGTACC2250.001718072123.5909844
GTGTATG3202.1138128E-523.2223761
CCGAGCA2752.0251182E-423.162069
CCCATAC3752.804998E-622.6473463
GTGCTAC2350.002212395422.5871141
ATAGCTC5305.142283E-922.033098
CTATACT4354.1355088E-721.9640224
GTATAGG2450.002818137621.665191
ATACTAG3954.4024328E-621.5006471