FastQCFastQC Report
Wed 20 Jul 2016
MG317748_forward_paired.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameMG317748_forward_paired.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences470299
Sequences flagged as poor quality0
Sequence length50-241
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGGCC500.002573833367.468553
GCGTGCG853.1608416E-452.916519
TGACGCG955.471615E-447.3463485
CCTTAGG3006.4446795E-933.7342721
GTACAGG3805.4569682E-1132.5506171
ACCACGC1400.003678734232.127888
GTCTATA3452.5142072E-829.334151
CTATGCG1600.00706716428.1118959
CCTATAC2855.50989E-627.6187063
TCGGGAC1650.00821225127.260023
CTATACT4605.11136E-1026.8896394
CAGGCGG4358.3182385E-925.850027
TATACTG6150.025.5978245
GCACTCC2651.0659793E-425.4598316
CCTACAT2701.21029494E-424.9883523
ACAGCGT2851.7466708E-423.6731788
TTAGGCA3351.9303896E-523.496514
CAGGACT2500.002268770722.4895154
CCAACGT3002.4722883E-422.4895154
TAGGACT2550.002545330722.0485464