FastQCFastQC Report
Wed 20 Jul 2016
MG038849_reverse_paired.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameMG038849_reverse_paired.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences883532
Sequences flagged as poor quality0
Sequence length50-241
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACACCG8000.023.4631025
TATACCG5303.276E-922.9162395
GTCTTAC3953.1360214E-622.362451
CAGTCCG4453.5326593E-722.3310439
GTATATA9650.020.595471
CCAGGAC3254.8072083E-420.3842353
GTCTAGG4407.983865E-620.0753821
TATACAG12200.019.910835
GTGTAGA9500.019.7584041
CTATCCT7904.1836756E-1119.5671444
GTCTACG4551.0664842E-519.4135571
TAGAGTG4008.7292356E-519.3225565
ACCGTAC3457.1877375E-419.202548
GCCTTAT7003.8908183E-918.9282171
CCGTGCA4701.4107371E-518.7939749
GACAGTG5402.2625863E-618.4024337
TAGGCTC3609.568394E-418.4024335
GTGTATG8451.10958354E-1018.2935431
TCCTATA6654.4577973E-818.2640692
GCCTATA4301.5198631E-417.9744721