FastQCFastQC Report
Wed 20 Jul 2016
MG038849_forward_paired.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameMG038849_forward_paired.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences883532
Sequences flagged as poor quality0
Sequence length50-241
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTGCG2057.1079767E-427.4485639
ATAGGAC3704.9312803E-827.3743743
GGGAGGG2159.387677E-426.1718857
GTCCTGG2701.20479854E-425.008691
CCTAGCT3052.7523006E-422.1388423
CCTGGGG3103.0721867E-421.7817632
CCTATAC6359.131327E-1021.2672333
GTATAGG5909.064024E-920.9818691
GTGTATA8650.020.816481
TATACTG11050.020.3690685
GAGAGCG3355.185913E-420.1562587
CCTGCAC5059.923588E-720.0564753
TCCGCCG5000.004501312519.98358235
TGTACCG8503.6379788E-1219.8598425
CTAGGAC4058.2954815E-519.4512043
ACCGTCG2900.00534092419.4032968
AGCGTGC3506.965412E-419.2924188
GTACACT5301.576811E-619.1104154
ACCGCTC4751.31133365E-518.9539558
TACACAG5401.8856681E-618.7565175