FastQCFastQC Report
Wed 20 Jul 2016
MG038315_reverse_paired.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameMG038315_reverse_paired.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences702895
Sequences flagged as poor quality0
Sequence length50-241
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGGAC2501.9681465E-938.093713
GCGTGCG1300.003447554832.5587239
ACCGTGC2903.0707997E-729.1905788
TCAGGGC1600.00950979126.4539664
TAGGACT4057.4378477E-926.1273734
CCTAAGG2100.001171984725.1942522
GACCGTG3051.4962314E-524.2856087
AAGGGCG2752.071498E-423.0870935
ACCGTCC2300.001990521823.0034478
GCCTTAT5457.381459E-921.3573281
CTGGCCA2550.003623150320.7482074
GTCTAAC3104.6555756E-420.480491
CGTGCTT6150.0039405620.444832235
CCGTGCA4156.951859E-620.3982379
CTAAGAC4156.951859E-620.3982373
GTGTAGG4708.9907553E-720.2626111
TAGACTC2650.004527095719.9652565
GCCTAGT2700.00504402219.595531
TAAGACC3859.020371E-519.2392464
CAGAGGC2750.005608421319.2392444