FastQCFastQC Report
Wed 20 Jul 2016
MG038315_forward_paired.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameMG038315_forward_paired.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences702895
Sequences flagged as poor quality0
Sequence length50-241
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGGTC1550.006030888329.0415744
GTCTAGG2855.481341E-627.6404461
CCCTATG2159.387224E-426.1711861
CGTAGGG2200.001073482625.5763872
TAGACCG2350.001576610823.9438515
GTCCTGG2901.956062E-423.283331
GCAGTGC3402.1535843E-523.1691978
TCCTATA6353.6379788E-1123.0388892
CTATACC4002.9637595E-622.5072214
TTAGTAC5054.029971E-822.2843783
GTACTAC3052.75202E-422.1382521
TAGTGGG2600.002837424921.6415584
GCCTTAG3153.422668E-421.4354481
CCTATAC4305.548067E-620.9369493
TAGTACC3254.226811E-420.7758964
CTATAAG4356.1319643E-620.6962952
TCTACAC7302.382876E-1020.0406743
GACTGTA4508.2209335E-620.0064187
TCTAAGG4559.044452E-619.7865682
GTACAGG6352.109482E-819.4944441