FastQCFastQC Report
Wed 20 Jul 2016
MG019920_reverse_paired.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameMG019920_reverse_paired.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences652130
Sequences flagged as poor quality0
Sequence length50-241
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTCCG1652.7110236E-432.3576169
GGCCTAT1703.2298756E-431.4059221
ACCGTCG1855.300433E-428.8594978
TATACAG8750.025.6272345
GCCGTCC2250.001661446623.7289188
ACAGTGC2852.480786E-422.4800288
GTCCTGG2400.002417977722.2458611
ACAGTGT4905.104266E-821.7918648
CCGACCA2500.003064466421.3560289
TATACGG4505.424863E-721.3560265
GTATAGA5609.08949E-920.9746691
GTGTAGG3655.5816476E-520.478381
GTACTAG3655.5816476E-520.478381
TATACCG4708.239058E-720.447265
CCTGGAC3256.0212763E-419.7132553
GTATAAG7654.3655746E-1119.5414621
ATAGGAC2800.00590549819.067883
TAGAGAG2850.00654099218.7333565
CTGGACC3458.99822E-418.5704574
TGGACCG3509.911263E-418.3051645