FastQCFastQC Report
Wed 20 Jul 2016
MG019920_forward_paired.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameMG019920_forward_paired.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences652130
Sequences flagged as poor quality0
Sequence length50-241
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGTAGG1500.005284777429.8367311
GTCCTAG2906.562057E-627.0073871
TAGCCTG2159.708427E-426.0204055
GTGAGCG2701.2527418E-424.8639459
TATAGGG2952.2835641E-422.7568323
TATACTG6905.456968E-1222.7018625
CTAGACT4453.10647E-722.6289834
ACACGGT3002.5586865E-422.3775486
CCCACAC2500.00233648422.3775483
TGCACCG5504.307367E-922.3775485
CAGTGCC2550.002621245621.9387749
GTCTAGC4103.859943E-621.8317571
AGTACTG4255.2699725E-621.0612225
ATTATAC11850.020.772413
CCTAGAC3254.3943943E-420.6561993
GTATAAG7051.6189006E-1020.6317841
GAGCTTA3855.8734862E-520.3432279
ATACTAT9450.020.1279536
ACCGAGC2800.004507514619.9799568
TAGACTG3957.154056E-519.8282075