FastQCFastQC Report
Wed 20 Jul 2016
MG017536_reverse_paired.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameMG017536_reverse_paired.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences808675
Sequences flagged as poor quality0
Sequence length50-241
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCTAT3801.8189894E-1233.9332161
CTTAGAG4056.31735E-926.532143
AGGTCGC2100.001071622425.5845648
GTATAGA7300.025.0238081
GTCGTGC2300.001820508923.359828
CTCTTAG5102.8740033E-923.1766051
CCTAGGA3802.8364393E-622.6221432
ATAAGAC9500.022.622143
GTCCATA8750.022.1050641
ACTATAC5557.628842E-921.297423
ACCGTGT4054.9289956E-621.2257148
GTATATA8250.020.8397921
GCCTTAA3104.1976484E-420.7977731
GACCGTG3104.1976484E-420.7977737
CTAGGAC2600.00370953820.6644573
TACACTG7352.1827873E-1120.4676515
CTATACA7903.6379788E-1220.4028824
TACTCAG5551.7798084E-719.3612925
CTATAGG3908.853007E-519.2868252
GTCTAAG3908.853007E-519.2868251