FastQCFastQC Report
Wed 20 Jul 2016
MG017536_forward_paired.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameMG017536_forward_paired.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences808675
Sequences flagged as poor quality0
Sequence length50-241
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCAGGGC1859.109826E-636.506818
CCGTGCG1600.007034906728.1406679
GGACCCA1950.00914673126.666025230-234
GTCCTAG3806.3724656E-826.6595781
CTATACT7250.026.3940034
TACACCG8550.026.3304485
ACTGGGC2651.0595194E-425.4858863
CCTAGCT2851.7361055E-423.6974033
GTCTAGG3351.9173849E-523.5205551
AGTGTCC2600.00283390621.6466668
CCTATAC4204.517242E-621.440513
GTACTGG5807.4232958E-921.3480911
GGGCACA2650.003165007321.2382376
ATACTGT11750.021.0755636
GTATAAG8101.8189894E-1220.8449381
GTGTTAG5509.9165845E-820.465941
TACACCC2750.00392287220.465944
GGAGTGT3304.6787836E-420.4659396
ACCGTGC3355.1780796E-420.1604778
GTTATAC7953.45608E-1119.8223553