FastQCFastQC Report
Wed 20 Jul 2016
MG016178_reverse_paired.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameMG016178_reverse_paired.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences846773
Sequences flagged as poor quality0
Sequence length50-241
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACTC2501.3614935E-424.562145
GTGTATA7250.023.9974921
GTATAGG5004.0163286E-922.5152951
CTAGACT4553.653804E-822.4928024
TAGACAG7800.022.3053635
TACACAG6507.2759576E-1222.0429445
TGGACTG6158.54925E-1121.633325
TAGGACC3404.4835615E-521.0704634
GTATATA9350.020.7968221
CCTATAC6001.4697434E-920.468453
TAGCACC6453.614332E-919.0404194
GTCTAGA4901.8464671E-618.7975561
CCGTGGA3308.8809466E-418.6076832
ACTAGAC7759.458745E-1118.4876333
ACAGTGC3901.2868666E-418.3691228
CACCGTG3359.824901E-418.3299567
CCTACAC5052.46339E-618.2392123
TATAAGG9550.018.217992
TATACTG9153.6379788E-1217.8959125
GTGCAGG4602.253375E-517.7986531