FastQCFastQC Report
Wed 20 Jul 2016
MG016178_forward_paired.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameMG016178_forward_paired.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences846773
Sequences flagged as poor quality0
Sequence length50-241
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATATAC9250.026.9963573
TATATAC9750.024.4983673
ACAGTGG5401.8735591E-1024.1271788
CCTAGCT2600.00349388420.879293
GTCTAGG3705.455057E-520.5407071
ATATACA14350.020.4282384
CCTATAC5901.369699E-820.2422943
CCCTCTG2700.00434749120.1059827
AGGGCCA2700.00434749120.1059825
TATACAC8255.456968E-1219.740423
CTATACT8355.456968E-1219.5040074
TAAGGTG5051.3996614E-619.349525
TATACAG10150.019.2542025
TAGGACC3407.295904E-419.1598194
TTATATA20550.019.0199662
GTATATG9800.018.8339731
GTATATA9301.8189894E-1218.6791061
GGCATAG4101.2003239E-418.5367361
TACACAG7156.2354957E-918.2219245
GTCTAGT6003.623627E-718.0953831