FastQCFastQC Report
Wed 20 Jul 2016
MG016017_reverse_paired.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameMG016017_reverse_paired.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1143712
Sequences flagged as poor quality0
Sequence length50-241
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGGCT4152.3293978E-823.4700474
CCTATAC7500.023.3761653
TACACCG9300.020.9463865
TACACTG8950.020.6772425
TAGGGGG2450.00464545519.8776935
CTATACT10750.019.0271134
TGTAGGC4651.7974289E-618.8517483
GTATAGG6304.994945E-918.5525131
TATATAC11650.018.3932633
CACCGTG3200.001007361518.262637
CTAGGAC4802.4348956E-618.262633
TAGGCTC3751.3927324E-418.1814635
GTATAGA10800.018.0371671
GCCTAGC2750.00904942617.7092178
TATAGGG5003.594976E-617.5321252
CCCTATA4452.5943296E-517.5102372
CTACACC12250.017.4923694
CTATAGG4502.8556202E-517.315683
TCTACAC10150.017.2730313
ACCGTGC3400.001512602417.1883588