FastQCFastQC Report
Wed 20 Jul 2016
MG016017_forward_paired.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameMG016017_forward_paired.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1143712
Sequences flagged as poor quality0
Sequence length50-241
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGGGC2155.3396907E-528.1874453
CAGGGCC1500.00871267326.9346689
CTTAGGG3508.212737E-825.9727153
CCTACAC7000.024.5297873
TAGGGGG2100.001537555824.0488135
TACACTG8650.022.1860715
TAGACTG5351.02099875E-820.7673845
CTATACT9800.020.6132664
TGTGTGC4458.325296E-720.427988
GTCTAGG4951.02425474E-720.4050521
GTACTAG6703.2196112E-1019.5979861
ACAGGGC4151.04014125E-519.4708448
GGGGAGG2600.00530897419.4240386
GCCTTAT8451.8189894E-1219.1252081
TCTAGGG3751.0541445E-418.8542672
CTTACAC7507.094059E-1118.8542673
GTGTGCT4351.5608337E-518.5756349
CTACACA8801.8189894E-1218.3645484
TATACTG12700.018.2922465
GAGTGCG2800.0081430218.0366087