FastQCFastQC Report
Wed 20 Jul 2016
MG015602_reverse_paired.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameMG015602_reverse_paired.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences809220
Sequences flagged as poor quality0
Sequence length50-241
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACCG3301.5643309E-930.2368895
CCTAGAC3153.3238393E-828.5090663
GTACTAG3951.0653821E-825.2611981
GTCCTAG2851.394657E-524.5077931
GTACTAC3002.0744232E-523.2824041
GTGTAGG4053.780824E-722.1737171
GTATAAG7900.021.472021
GTATAGG4306.727714E-720.8845481
TAGACTG4008.403476E-619.9563475
TATAGGG3557.5956516E-519.675272
CTAGACT3056.198848E-419.6291924
GTATAGT6653.4197E-1019.5062031
GTCTTAT8351.8189894E-1219.1198521
TAGACTA5853.274181E-818.7623775
TCCTAGA5853.274181E-818.7623772
CCCCTGA3208.5620384E-418.7090741
CCTACAC4801.9314957E-618.7090743
TGGGCGC2700.00708135818.47818
GTTCCCG3259.502026E-418.4212449
GTATATA6004.375579E-818.2933181