FastQCFastQC Report
Wed 20 Jul 2016
MG015602_forward_paired.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameMG015602_forward_paired.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences809220
Sequences flagged as poor quality0
Sequence length50-241
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAAG7450.026.9650521
TAGACTA6351.8189894E-1224.6059045
TACACAC4352.558172E-723.0907613
CTAGACT2952.3236405E-422.6993924
GTCCTAA5954.4929038E-1022.5086421
CGTATAC5003.9623046E-822.321073
CCTATAC4053.548921E-622.0455023
GTCCTGG2700.003706212220.6676581
TACACAG6501.3533281E-920.6040635
TATACTG8203.6379788E-1220.4156135
TTAGGGC4407.2755756E-620.2918824
GGATAGG3957.29717E-519.7781621
GTCCTAT4559.719972E-619.6229171
TATACGG5852.0786865E-719.0778375
ACACACT3000.006815834418.6008934
CTCTAGA5452.2296972E-618.4302431
TATACCG4251.2798545E-418.3820575
TCTATAC7907.6579454E-1018.3654373
CTATACT7305.7298166E-918.3460854
TATAGTG6805.0878953E-818.0538085