FastQCFastQC Report
Wed 20 Jul 2016
BU845243_reverse_paired.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameBU845243_reverse_paired.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences848374
Sequences flagged as poor quality0
Sequence length50-241
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGAGGG1803.6477813E-430.762147
GTATAGG5250.029.5316541
CCTATAC5451.6552804E-1024.3839363
CTAGGAC3751.9456565E-623.6253243
CAGGACC2851.938892E-423.3144664
ACCGTGG3503.0510002E-522.148748
ACCGCCC3053.0683455E-421.7856468
ACCGAGC2600.003114934521.2968678
TAGACGG5859.888026E-920.8236055
TAGGACA7451.8189894E-1120.81094
CCAGGAC2800.00478482719.7756633
TAGGCTG5651.5640035E-719.6006555
TATACAG10900.019.303955
CTAAGAC4651.2537899E-519.052683
ACAGCGC3507.7608845E-418.9846368
AGGGTGT2950.00646894718.770126
AGGACCG3558.537413E-418.7172475
GTATACT8901.0913936E-1118.664674
GTCTTAT9551.8189894E-1218.5539191
TAAGACA9701.8189894E-1218.2670044