FastQCFastQC Report
Wed 20 Jul 2016
BU845243_forward_paired.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameBU845243_forward_paired.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences848374
Sequences flagged as poor quality0
Sequence length50-241
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCTATA3701.5440291E-624.2615622
GTATAGG5054.1585736E-822.2197481
CTATATA8700.021.9260394
GTACTAG5256.2720574E-821.3732811
CCTATAC6359.458745E-1021.2049883
GTATATA9700.020.8224241
TAGACCG3456.4481486E-419.5147365
GTGTAGG6352.1826054E-819.4379061
TCTAGGG3557.8150106E-418.9650272
GTGTATG6553.1150194E-818.8443831
ACACTGC4201.1215295E-418.7016226
ACCGACC3050.007268096318.3950378
TATACCG6103.049663E-718.3950375
TATACTG10500.018.167295
TTAAGAC5602.7449587E-618.0337073
GTACTAT5602.7449587E-618.0337071
ATCTAGG6253.9355473E-717.9535561
ACCGCTC3800.001234220617.7173258
GCACAGT3800.001234220617.7173256
TTAAGGC3800.001234220617.7173254