FastQCFastQC Report
Wed 20 Jul 2016
BU814330_reverse_paired.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameBU814330_reverse_paired.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences984368
Sequences flagged as poor quality0
Sequence length50-241
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACCCGT1550.0088687126.8364376
AGTGTCC3106.409791E-726.8364378
CCTATAC5652.1827873E-1123.9271763
TATACCG6253.6379788E-1223.2940255
ACAGTGC4304.5234628E-721.7655968
CCTAGAC3004.1979106E-420.7982393
CCGTGCA4608.6500404E-720.3461029
CACTGTG4107.2810562E-620.2909627
GTCTACG4701.0632539E-619.9132061
GTGAGCG2650.00500770519.620989
TAGGACC2650.00500770519.620984
ACCGTGC4259.931748E-619.5748128
GTCTAGA5952.4774636E-819.2252621
TAGGCTA3809.3295464E-519.1562715
CTATACT8801.8189894E-1218.9074884
GTCTATG7808.185452E-1118.6650851
CAGAGCG3358.8260876E-418.6252869
GTGGGTG3409.749646E-418.3513859
AGGACCT3450.001075355618.0854245
TACACTG8151.546141E-1017.8635185