FastQCFastQC Report
Wed 20 Jul 2016
BU814330_forward_paired.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameBU814330_forward_paired.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences984368
Sequences flagged as poor quality0
Sequence length50-241
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGGTC1803.542837E-430.9163345
CTAGGAC3751.8633691E-623.7437463
TAAGACC4852.9558578E-822.9482064
CTATACT10700.022.8838654
CCCTATA4903.2952812E-822.714042
GTCTAGG3603.64913E-521.6414341
TTAGGGT5155.5808414E-821.6114184
CTGTGCA6207.7852746E-1021.5417049
CACGCCC2600.003026237721.4036167
ACACGCC3153.6895034E-421.199776
GTACTAG6451.2732926E-920.7067551
TAGAGAG4306.1093087E-620.7067535
GACCGTG4407.4533637E-620.2361477
GTCCTAG3355.588684E-419.9341161
TATAGGG3957.455175E-519.7238392
TAGGACC4008.211728E-519.477294
TATACAG13800.019.3563125
ATATAGG7554.3473847E-1019.1640321
TATAAGA16450.018.9444772
ACCCGTC2950.006285582718.8642027