FastQCFastQC Report
Wed 20 Jul 2016
BU795895_reverse_paired.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameBU795895_reverse_paired.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences700728
Sequences flagged as poor quality0
Sequence length50-241
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCCC1501.300177E-436.668583
GTATAGG4600.031.088581
GTGTGCG1550.006739387828.388589
AGTGGGC1600.00786906527.5014388
GCCTAGC1650.0091433326.6680581
TAGGGCT2150.001071927425.5827314
CTAGACC3701.8341689E-623.7850254
CTATACC3953.2370463E-622.2796464
ACCGTGC2500.002578583322.0011488
CTTACAC5156.308437E-821.3603383
AGTGTGC2600.003237523421.1549518
TACACAG4706.1915307E-721.064935
CCCTATA3755.4662298E-520.5344072
CCTAGAC2700.004028911220.3714353
AGCTAGG2700.004028911220.3714351
GTCATAG4459.08497E-619.7763141
GTGGTAC3406.677994E-419.4127773
GTGTACG2850.0055083819.2992521
GTATATA8801.0913936E-1118.7509781
TAATACA13900.018.202394