FastQCFastQC Report
Wed 20 Jul 2016
BU795895_forward_paired.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameBU795895_forward_paired.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences700728
Sequences flagged as poor quality0
Sequence length50-241
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGTGC1300.002621365534.43076
GGGTGCT1550.006200278628.8773617
ACCGACC2006.3595915E-427.9749438
CCGACCT1650.00841299127.1272189
GTCCACA4901.1295924E-925.1203581
GTCCTAG2801.602386E-423.9785251
TCTACAC7950.023.9282533
TAGACTG3801.9938307E-623.5578485
GTATAAA14250.021.9873241
GTAGACC3603.4987854E-521.758294
TACACCG6258.021743E-1021.4847565
GTATAAG7652.1827873E-1120.4783921
GGGTTAC3304.8680205E-420.3454131
CTAGGAG3906.482009E-520.0845763
CACCGTG2800.004505042519.9821037
TATACCG4007.874782E-519.582465
GAGTGCA3507.2355533E-419.1828179
TGGACTG5301.664188E-619.0018485
CTACACC8357.6397555E-1118.7616394
TAGACCT4801.5066886E-518.6499635