FastQCFastQC Report
Wed 20 Jul 2016
BU768105_reverse_paired.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameBU768105_reverse_paired.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences837725
Sequences flagged as poor quality0
Sequence length50-241
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTAGG3107.2759576E-1238.5017471
CTAGGGT1804.1114737E-430.1402593
ACAGGCC2305.1879477E-528.3056348
GCGCCGC1600.00841540927.126236
GTACTAC3758.000461E-826.0411831
CAGGCCA2651.359592E-424.5671549
TAGTACT5301.4915713E-1024.5671544
TCTAGGG3201.7871893E-523.7354532
TACACTG6401.8189894E-1223.7354535
CTAGTAC5102.568413E-923.4030233
GTATAGG4252.6826638E-722.9775141
GTGTAGG6153.8198777E-1122.9359991
TAGACAG6805.456968E-1222.3392495
TATACCG6356.002665E-1122.2136065
CAGGGCA2450.002483968922.1438644
CATACAC7400.021.9942443
GTATATA7451.8189894E-1221.8466281
TAAGACC4206.2083545E-620.6676044
CTACACT6401.5861588E-920.3446734
GTCCAGG3204.8703104E-420.3446731