FastQCFastQC Report
Wed 20 Jul 2016
BU768105_forward_paired.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameBU768105_forward_paired.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences837725
Sequences flagged as poor quality0
Sequence length50-241
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGGGCC1203.065291E-546.8520669
CCGCGCC1058.96352E-442.8361789
GTCCTAG4101.3435601E-724.6830431
GCGAGCG3301.7207254E-523.8519619
TAGAGTG4604.0785199E-722.0001035
CTAGAGT4103.699257E-621.9404814
GTGGTCC2600.00285124721.6240319
ACTGTGC4755.554102E-721.305368
CTGTGCG3203.828526E-421.0834319
ACAGTGC4305.589225E-620.9199948
GTGCAGG6501.2332748E-920.759071
AGGTCTC2750.003946828720.444547
TTATACC10300.019.6505784
GAGGTCT2900.00536659519.3870646
CTCCTAG4109.1732E-519.1979221
GTATTAG15900.019.0944291
GTCTATA5301.5892929E-619.0944291
GTATAGG5301.5892929E-619.0944291
TATACCG5902.101242E-719.0584725
TAGTCCC2950.005923738219.0584727