FastQCFastQC Report
Wed 20 Jul 2016
BU767578_reverse_paired.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameBU767578_reverse_paired.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences976400
Sequences flagged as poor quality0
Sequence length50-241
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCCTGG1250.002768037434.0518231
CTAGGGC2052.702612E-531.1449623
GTCTAGG3551.718945E-929.9751951
GTAGGGC1600.00925463726.6029873
TAGCCTG3403.3197997E-521.9083425
TTGGCCC2953.2080334E-421.6431084
TCTATAC9050.021.1648083
CTATACT9650.020.9515764
GCCGTGC2600.003925782620.4638358
CTAGTAC5851.5656042E-820.0090833
ACACTGC4801.0434524E-619.952246
CAGGCCA2750.005430758519.3476289
GTATATA12300.019.0330311
CTAACCT2800.00602659719.0021343
TACCTCG2800.00602659719.0021345
CTAGACC5051.6972135E-618.9645044
CAGAGCC3408.352968E-418.778589
TCTAGGG3459.213937E-418.5064262
CTCTTAA8651.2732926E-1118.4529381
CTATAGG3500.001014872518.2420481