FastQCFastQC Report
Wed 20 Jul 2016
BU767578_forward_paired.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameBU767578_forward_paired.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences976400
Sequences flagged as poor quality0
Sequence length50-241
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACAGTGC3805.638867E-1132.527048
TATACTG13150.023.0713735
GTCTAGA6353.8198777E-1123.0040911
CTTACAC6551.369699E-920.5861643
ATACGGT9900.020.4302086
TATACAG14050.019.9939755
TAGGGCA2900.00538901719.3734724
TACACAG7654.5656634E-1019.0948985
GGACCGT3557.7432924E-418.991466
CTATAGT4851.590446E-518.5346224
AACCGAG4251.2152454E-418.5073647
CAGTGCA6804.710273E-818.1768749
TTAGACT7456.7830115E-918.0992434
GTTATAC11200.018.0588443
GTCTTAA6855.1220923E-818.0441971
CAGCGCA3850.001334927417.5116089
TCTAGGT7158.3595296E-817.28713
GTATATA12600.016.9441011
TAGGGCT4000.00172427616.8549214
ACCGTGC4000.00172427616.8549218