FastQCFastQC Report
Wed 20 Jul 2016
BI123566_reverse_paired.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameBI123566_reverse_paired.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences844340
Sequences flagged as poor quality0
Sequence length50-241
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTATAC5750.026.9378283
GTATAGG4851.1459633E-925.0930421
GTGTAGG5751.2732926E-1125.0136971
GACTACG3902.7587103E-622.6948747
ACAGAGC3953.0813335E-622.4075978
GTATAGT7057.2759576E-1221.970571
TAGCACT6351.1277734E-920.9078754
GTATATA10850.020.394011
CTATACT7652.5465852E-1120.2473894
TATACAG12050.020.1993795
GTCCTAG4959.644773E-720.1159111
CACCGTG3305.2561646E-420.1159117
TAGGTAG5001.0620952E-619.9147515
GTAGGAC5001.0620952E-619.9147513
CCCTATA3355.8166863E-419.8156742
GTCCACA4509.526337E-619.668891
GTACCGC5651.5798832E-719.581866
GTCTAAG4551.0479896E-519.452751
TATAGGG3457.0905057E-419.2413062
AGCACTC4601.1516393E-519.2413065