FastQCFastQC Report
Wed 20 Jul 2016
BI123566_forward_paired.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameBI123566_forward_paired.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences844340
Sequences flagged as poor quality0
Sequence length50-241
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCTGG3152.7830538E-1035.5314451
CCAGGAC3352.0037942E-523.3871173
GTCTAAG3902.4951387E-622.9587781
CTTACCC2952.2793272E-422.7642143
TACACCG7801.8189894E-1221.5238555
TATACAG10950.021.4648865
CCCTTAG2800.00450014119.9864391
CTGGACC5251.5174228E-619.1869814
ATAGGAC4751.3746556E-518.8503653
AGAGTGG5452.1699707E-618.482876
CTTAGAG3700.001049827418.1498473
GCCGTAC3700.001049827418.1498478
CTAGCAC3700.001049827418.1498473
TATACCG6203.7154678E-718.0522655
CTATGCG3750.001148777317.9078469
TACCCTG3750.001148777317.9078465
TAGACAG6304.3944965E-717.7657225
ACCTAAG5052.3288698E-517.7305431
GCCTTAC4451.7807476E-417.606031
GTATAGA9602.910383E-1117.4881321