FastQCFastQC Report
Wed 20 Jul 2016
BB420982_reverse_paired.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameBB420982_reverse_paired.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences595552
Sequences flagged as poor quality0
Sequence length50-241
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCTAG1808.887706E-636.6361051
GACCGAG1753.2316794E-431.4023767
CTAGACA2653.7386108E-629.0323874
TATATAC4951.5643309E-924.424073
AGACAGT5202.7685019E-923.2498386
TAGACAG3452.8927021E-522.3002385
TAGTACA4453.6884012E-722.2286474
ACCGTGC4003.636651E-621.9816658
GCTAGAG2550.002907067321.5506523
TATACAG7701.8189894E-1221.4107135
GTATATG7251.2732926E-1121.2236771
CTATACA5257.7980076E-820.934924
GTATACC2650.00363344320.737423
GTGTAGG4858.440056E-720.3953591
GTACTAG2700.00404893320.3533921
TAGGACC3254.955907E-420.2907684
CTTAGAC3806.0922852E-520.246273
CTATCCC2750.004502660619.9833323
TTAGACC5551.4016223E-719.80334
ATGCTAG3356.0787256E-419.6850721