FastQCFastQC Report
Wed 20 Jul 2016
BB420982_forward_paired.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameBB420982_forward_paired.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences595552
Sequences flagged as poor quality0
Sequence length50-241
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGCGC1854.0106883E-430.2664368
TAGTCCC1500.00526182929.8628834
ACAGTGC4901.1186785E-925.1396718
CTGCGTC2300.001431049324.3447429
TCCTATG2450.002066900722.8542482
ACTGTCC2450.002066900722.8542488
GTATAGA6055.293259E-1022.212061
CTAGACC4553.8147846E-722.151044
TACAGTG5205.7822035E-821.5357347
TCTATAG5205.7822035E-821.5357343
CCTATAC3805.274553E-520.6289653
TAGTACG2750.004040115520.3610574
TACACCG5051.0392359E-619.9578675
TATACTG4508.573012E-619.908595
GTATATA4651.1378519E-519.2663761
TACACTG4651.1378519E-519.2663765
ACACCGT4151.03822895E-418.8891746
CTACGCT3659.5418154E-418.4086274
CCGACTA3659.5418154E-418.4086279
TATGCCG5552.5655572E-618.1598635