FastQCFastQC Report
Wed 20 Jul 2016
BB417939_reverse_paired.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameBB417939_reverse_paired.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences975460
Sequences flagged as poor quality0
Sequence length50-241
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCTAGG1552.763453E-432.2541471
AAGGGCC1857.7868387E-427.0237435
ACCGCTC5251.4551915E-1124.7588988
TAGACTG4252.2699169E-823.5265545
TAGACGT5659.295036E-1021.2363575
TATAGGG3805.2986325E-621.0500762
ACACTAC3805.2986325E-621.0500766
AGACCCG2400.003541659820.8308035
ACCGTGT4357.373692E-720.6871418
CACCGTG4408.229108E-720.4520637
CTATAGG3957.4070504E-620.2507041
TATACCG6001.9663275E-919.997575
GTATAGA8150.019.6295171
GTACTAG5652.1467713E-819.4666611
GTACTAT6202.9558578E-919.352491
CCAGGAC3157.5972424E-419.0453053
GTCTAGA4751.7139482E-618.9450681
CCCTATA3701.0275809E-418.916621
GGGCTGC2650.00628279518.8656316
TCCTATA8001.0913936E-1118.7477232