FastQCFastQC Report
Wed 20 Jul 2016
BB417939_forward_paired.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameBB417939_forward_paired.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences975460
Sequences flagged as poor quality0
Sequence length50-241
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCCCTC2450.002075403222.83998
CACCGTG4503.455043E-722.38317
TAGACAG8300.021.5740725
TCCTAAG7401.2732926E-1121.1732032
CAGGCCT2650.003273094321.116134
TGGACCG4255.2630767E-621.0664485
TAGACTG5701.5365913E-719.6342965
GTATAGC7554.0381565E-1019.270221
GTACATA10000.019.0256351
TAAGGGC4201.1445632E-418.6525824
GTCCTAA8551.07320375E-1018.3253461
GGGCTGT5552.5844238E-618.148466
GTATATA10500.018.1196521
TAAGACC5002.1398751E-517.906484
TAGAGTG4401.6345606E-417.8047375
CCCTTAC4401.6345606E-417.8047371
TATTAGG15200.017.6708682
TATACAG12750.017.5553725
ACACTGT8301.4279067E-917.5289346
CCACACT6405.189413E-717.4867974