FastQCFastQC Report
Wed 20 Jul 2016
BB416467_reverse_paired.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameBB416467_reverse_paired.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences527421
Sequences flagged as poor quality0
Sequence length50-241
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCCCGG150.008453045136.716227
ACCGCAC1450.006857918628.2861168
GTGTAGG2758.551782E-626.1003681
AGAGGGG2150.00161417323.8458548
GTATAGA5252.7102942E-1023.4370651
ATAGGAC3152.4483876E-522.7860373
ATAGTAC3602.6611688E-622.7860363
CACCGTG2300.002389269422.290697
GTATATG6651.0913936E-1121.5867711
TATAGGA5256.886694E-921.4839762
CCTTAAC2400.003058808421.3619082
GGCTAGA2500.003875361520.5074331
CTATGTG2600.00486295519.7186859
TAGTACC2600.00486295519.7186854
CTATACC3156.4079236E-419.530894
GGTATGG4201.0111642E-519.5308881
TTAGACT4751.3443969E-619.4280954
GTATAGT3759.379067E-519.140271
TATATAC6453.5215635E-919.0766813
TATAGAG4851.6412541E-619.0275152