FastQCFastQC Report
Wed 20 Jul 2016
BB416467_forward_paired.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameBB416467_forward_paired.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences527421
Sequences flagged as poor quality0
Sequence length50-241
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGTGGG650.00739727251.6783077
TAGGGTC1150.001431220738.9459725
GTCCTAG2203.089355E-530.537181
TAGCGTC1550.00618002928.8953959
GGAGCGC1650.008385560527.1441618
GGACTAC2300.001432034524.3412326
GTCTAGG2350.001623075623.8233321
TATAGTG4701.9826984E-823.8233325
GTGGTCT2400.001834611123.3270119
TATACAG7250.023.1661385
GTATATA6606.548362E-1122.0546321
GTTACAC2650.003261961721.126353
CTTATAC6501.2969394E-920.6713243
TATAGCG3906.447086E-520.0971185
CTACACA3906.447086E-520.0971184
CTTACAC3355.362351E-420.054273
TCTATAC5251.5093174E-619.19483
GTCCATA6452.6704583E-819.09561
GTCCTGC4201.1390632E-418.6616121
ACTCTGT3659.549088E-418.4059738