FastQCFastQC Report
Wed 20 Jul 2016
BB412635_reverse_paired.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameBB412635_reverse_paired.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences602271
Sequences flagged as poor quality0
Sequence length50-241
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACCGCG1350.007100439628.086127
CCGCTCT1850.001062907925.6190999
GTACACA2701.3644722E-524.5753561
ATAGTCC3352.8187897E-622.6365768
TATACGG4654.2055035E-922.4235955
GAGTAGG2552.6162487E-422.3036841
TATGCCC2200.002912839221.5433313
ACTGTGC2200.002912839221.5433318
GTATACC3808.399138E-619.9559293
TAAGGAG6703.45608E-1119.8070055
GTATAAT8350.019.2986981
CTAGAGC2500.006105173418.9581323
GTATAGA6104.6748028E-918.6473431
GGGTATA2550.006844567618.5864031
TATACCG3601.2530935E-418.4315175
GTATATA6758.2945917E-1018.255981
TATAGAC4252.2040123E-517.8429473
ACTGAGC3200.001207859317.773258
GTATTAG10800.017.5538251
GTCTTAT6651.3611498E-817.1050821