FastQCFastQC Report
Wed 20 Jul 2016
BB412635_forward_paired.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameBB412635_forward_paired.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences602271
Sequences flagged as poor quality0
Sequence length50-241
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATATAG5200.032.4380535
TAGGCCT1550.006052294729.0198555
CTGTGTG1550.006052294729.0198559
CTTATAG4501.19398464E-824.9893173
GTCCTAT3201.3544033E-524.598861
ACCCAGT2350.001583231123.9259438
ATATAGG4302.1253072E-723.5364511
CTAGAGT2952.2068339E-422.8715783
CTAGACG4503.2950993E-722.4903854
GGATGCG2550.00254558122.04945
CCTTATA6901.1277734E-1021.1865962
CCCTACA3203.8252032E-421.0847361
CTACGGT3254.247235E-420.7603574
TACCGAG3254.247235E-420.7603577
GGGCTGA2750.00394414520.4458076
GTATATA7252.2009772E-1020.1637941
TATAGGC2800.004377822420.0807022
ATTAGAC5651.3294266E-719.9029983
TTAGACC5151.2052842E-619.6517934
TAGACCT4058.339385E-519.4361365