FastQCFastQC Report
Wed 20 Jul 2016
BB411906_reverse_paired.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameBB411906_reverse_paired.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences911379
Sequences flagged as poor quality0
Sequence length50-241
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGACTGC3503.092282E-1134.2551776
GTATAAG7700.026.894561
CCGCGTA2857.0311708E-626.7703939
GTCTCAC3302.1902802E-523.1198861
TAAGGCC2400.002146886222.707034
GTCTTAG6006.712071E-1021.7987481
GTATATG10700.021.3912961
ACTGTGC7102.5283953E-1019.9566028
ACCGTGC6051.7487764E-819.8170458
GCACTAG3857.189103E-519.8170451
CTTAGAC7804.1836756E-1119.562983
AGGGTGC4551.1926495E-519.1637346
GTACACT4009.6422875E-519.0739044
AGGACTG2950.007092166718.4735165
TAGTACT6503.9823135E-818.4450954
TATAGTA3559.502464E-418.4214785
AGAGTCC3000.00781404218.1656258
TAGGCTC4201.4016648E-418.1656255
GTGTTAG7358.365532E-917.7948971
GTATAGC6154.5089837E-717.722561