FastQCFastQC Report
Wed 20 Jul 2016
BB411906_forward_paired.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameBB411906_forward_paired.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences911379
Sequences flagged as poor quality0
Sequence length50-241
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGACA4350.036.1104354
ACGGTCC1600.00714699628.0500688
AGGCCGT2558.3390754E-526.4000666
TAGACAG5555.456968E-1226.2811475
CTACCCT2250.001245579424.9333934
CTATACT5051.5552359E-924.4396634
CCTAGAC5601.9645086E-1024.0429153
ACTGGCC2801.5740101E-424.0429158
TATACTG7600.023.6211135
TATAGTA4453.0267802E-722.6921922
TATACAC7107.2759576E-1222.1239993
CCTAGTA3052.8091008E-422.0721862
GGACCAC4103.7704176E-621.8927366
TAGGCCA2600.002887709521.5769775
TGTAGAC6951.2914825E-1020.9871033
TACACTG9150.020.8459535
ATAGTGC8700.020.6345358
ACCGTGC4508.44052E-619.9467168
CTAGGAT4508.44052E-619.9467163
GTATATG9150.019.619721