FastQCFastQC Report
Mon 18 Jul 2016
Undetermined_S0_L001_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameUndetermined_S0_L001_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences524501
Sequences flagged as poor quality0
Sequence length32-241
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC17490.33345980274584797No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACGG2554.7451977E-836.106585
TGGACCG1651.7453454E-434.8756685
GTATTAG6100.028.3007491
CTGGACC1650.007333129627.9005364
GTGTTAC2506.1246086E-527.621531
GACCGTG1700.00848297327.0799357
ACAGGGC1700.00848297327.0799358
CTCATGG2300.001220106625.0195031
AGACTAC2750.003447766420.9254046
CTATACT3404.923353E-420.3099524
TCTATAC3607.235279E-419.1816183
GTATAGA3200.00827871517.9827691
CTTATAT7156.34227E-817.706113
AAGACTA4702.183044E-417.141025
GTCATAT4903.0003837E-416.4413871
ATATAGT4500.003223122315.3452946
TTACACC4650.00400901914.85028654
CTAATAC6551.691742E-414.0567594
GTATAAA7704.406762E-513.4520451
AGACAAT7354.4849343E-412.5267726