FastQCFastQC Report
Mon 18 Jul 2016
Undetermined_S0_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameUndetermined_S0_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences524501
Sequences flagged as poor quality0
Sequence length32-241
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCCCTT1050.00886560649.360817235
GTATAGG2002.909892E-740.244081
AGCTCCG1250.001892041336.7945865
CTAGAGC1951.1287506E-535.379413
CTCTATG1500.00462375130.6621552
TATAGGA2804.0376235E-628.745772
GTACTGG2158.2764594E-426.7402521
GTGTTGG3558.691859E-725.9116821
GTGTATA2801.3318958E-424.639231
TACACTG3901.971457E-623.5862715
AGTACCG2550.002236456122.5457025
GGATAAG3703.5024997E-521.7535551
CTACACC4304.601303E-621.39224
TGCTATA4355.0859944E-621.1463132
CTATTAC3304.05051E-420.9060151
TATACTG3905.2552892E-520.6379875
TGGACAC2850.004263073720.172475
GTATTAG5951.8124047E-719.3248881
AGTACAG3708.753269E-418.6459055
ATACTGC4951.5528052E-518.5831246